Learn how moving beyond gene-level counts to full-length transcript analysis reveals isoform-level complexity, and explore the biology, workflows, and sequencing technologies that make it possible.

Explore how ArgenTag enables isoform-level single-cell analysis in human PBMCs using Oxford Nanopore long-read sequencing workflows.

From ArgenTag cDNA to library preparation using the Oxford Nanopore Ligation Sequencing Kit (SQK-LSK114). Discover our platform’s ability to resolve complex gene fusions and Philadelphia heterogeneity in K562 cells.

A library of 1,000 human PBMCs was prepared following ArgenTag guidelines and the LSK114 kit. The cDNA was sequenced on one PromethION P2 flow cell to evaluate the effect of sequencing depth on isoform-resolved single-cell data.

The Kinnex™ Full-Length RNA Kit (8-fold concatenation) and the Kinnex™ 16S rRNA Kit (12-fold) were each used to generate one library from the same ArgenTag cDNA. Each library was sequenced on one Revio SMRT® Cell.

Check out how to generate PacBio® Kinnex™  libraries after obtaining your ArgenTag cDNA.

Learn more about the features and applications of the ArgenTag Single-Cell Kit.

Find out about the foundational research behind ArgenTag’s barcoding technology.

Presentation showcased at the workshop “High-Resolution Insights from Single-Cell and Spatial Transcriptomics with PacBio HiFi Sequencing”, CSHL 2025