Fusion-Resolved Single-Cell Profiling using ArgenTag and ONT
Technical Note
From ArgenTag cDNA to library preparation using the Oxford Nanopore Ligation Sequencing Kit (SQK-LSK114). Discover our platform’s ability to resolve complex gene fusions and Philadelphia heterogeneity in K562 cells.
Human PBMC Single-Cell RNA
Assay Sequenced with ONT
(~1000 cells)
Dataset_ONT
Library preparation employed the Oxford Nanopore LSK-114 amplicon sequencing kit following ArgenTag protocol guidelines. The library was sequenced on a PromethION 2 Solo device using a P2 flow cell.
Single-cell of MDA-MB-453
cell line sequenced
with PacBio®
Dataset_PacBio
The Kinnex™ Full-Length RNA Kit (8-fold concatenation) and the Kinnex™ 16S rRNA Kit (12-fold) were each used to generate one library from the same ArgenTag cDNA. Each library was sequenced on one Revio SMRT® Cell.
